Sequencing at WSU
The Apple Genome Project in the Dhingra Lab is being generously supported by the Agriculture Research Center, Department of Horticulture and Washington Tree Fruit Research Commission. In addition, several corporate entities have extended their support to the project by providing demonstration units or modules of the instruments and relevant software.
Roche Applied Science is providing a demonstration unit of their highly successful 454 GS FLX system in support of the Apple Genome Project and the many other sequencing projects within the WSU research community. There are over 150 publications to support a wide variety of applications such as Whole Genome Sequencing, Resequencing, BACs, Rare Mutation and Quantification (SNPs/InDels), Metagenomics, Transcriptome Analysis, Methylation/Epigenetics and Small RNA. The unit will be on campus for a minimum of 3 months, with the long term goal of having a system at WSU permanently. In preparation of the arrival of the 454 GSFLX in June 2008, we are collecting project specific information from WSU scientists and the surrounding research community who wish to access this sequencing technology. Please fill out the form above for us to prioritize the projects. The information provided will be kept strictly confidential and utilzied only for scheduling the sequencing runs.
How to schedule use of the Roche/454 GS FLX: High Through Put Sequencing at WSU
Important Documents
Sample Data Sheet
Sample Preparation and Shipping Instructions
We expect to set up a procedure for transfer of funds in the coming days to cover the costs of the reagents and consumables. The reagents will be directly purchased by and shipped to the Dhingra laboratory to streamline the overall process. This will also allow for sharing of resources and reagent running costs. The anticipated cost of each sequencing project will be discussed and agreed upon before the authorization of transferring of funds. The primary goal of this collaboration between Roche and the Dhingra Laboratory is to enable and facilitate critical research within the WSU research community at an affordable cost.
To further discuss the logistics of your project, please contact Dr. Amit Dhingra directly at (509) 335-3625 by email at adhingra@wsu.edu
